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cell suspensions  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cell suspensions
    Cell Suspensions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 3305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fcr+blocking+reagent/FcR+Blocking+Reagent%2C+human/pm42521848-287-2-23
    Average 97 stars, based on 3305 article reviews
    cell suspensions - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Tracking B cell immunity during perturbation of hepatitis B infection induced by treatment withdrawal
    Article Snippet: Cells were stained with Fixable Live/Dead Dye (Life Technologies, Thermo Fisher Scientific) before incubation with saturating concentrations of surface mAbs diluted in 50% Brilliant Violet Buffer (BD Biosciences) and 50% phosphate-buffered saline (PBS) for 30 min at 4°C. .. In all instances, cells were stained in the presence of FcR-blocking reagent (Miltenyi Biotec). .. All samples were acquired on a Fortessa-X20 (BD Biosciences) and analysed using FlowJo (V.10).

    Article Title: Genomic profiling identifies actionable DNA-repair defects in a new cervical cancer model
    Article Snippet: .. Cells were treated with an FcR blocking reagent (130-059-901, Miltenyi Biotec) for 10 min at 4 °C prior to antibody staining. ..

    Article Title: A tumor profiling resource for ovarian cancer: insights into chemotherapy-driven heterogeneity and personalized treatment strategy.
    Article Snippet: .. Combined cells were split and incubated with FcR blocking reagent (Miltenyi Biotec, Adliswil, Switzerland) for 10 min at 4°C before staining for 30 minutes at 4°C with either the tumor or the immune antibody panels. .. Before acquisition on CyTOF (Helios system, Standard BioTools), the cells were resuspended in 1mL of nucleic acid Ir-Intercalator in 1.6% PFA/PBS for 1 h at room temperature (Standard BioTools).

    Article Title: A poly(I:C)/QS-21-based in situ vaccine synergizes with anti-PD-1 therapy to overcome tumor immunoresistance.
    Article Snippet: The single-cell suspension Cell Reports Medicine 7, 102932, August 18, 2026 e5 Article ll OPEN ACCESS was lysed with red blood cell lysis buffer (420302, Biolegend). .. Cells were incubated with FcR Blocking Reagent (130-092-575, Miltenyi Biotec) before staining. ..

    Blocking Assay:

    Article Title: Genomic profiling identifies actionable DNA-repair defects in a new cervical cancer model
    Article Snippet: .. Cells were treated with an FcR blocking reagent (130-059-901, Miltenyi Biotec) for 10 min at 4 °C prior to antibody staining. ..

    Article Title: Humans homozygous for rare or common hypomorphic IL23R variants are prone to tuberculosis
    Article Snippet: .. They were then permeabilized/stained by incubation overnight at −20°C in the permeabilization buffer from the Nuclear Transcription Factor Buffer Set (BioLegend) with an intracellular cytokine panel containing FcR blocking reagent (Miltenyi Biotec; 1:50 dilution), anti-IFN-γ-BV711 (Clone: 4 S.B3, 502540; BioLegend; 1:50 dilution), anti-TNF-BV510 (Clone: MAb11, 502950; BioLegend; 1:50 dilution), and anti-IL-10-PE/Dazzle594 (Clone: JES3-19F1, 506812; BioLegend; 1:50 dilution) antibodies. .. As a positive control, cells in a separate well were stimulated by incubation with PMA (Sigma-Aldrich; 25 ng/ml) and ionomycin (Sigma-Aldrich; 500 nM) for 1 h without GolgiPlug and then for 7 h with GolgiPlug (for intracellular cytokine staining).

    Article Title: A tumor profiling resource for ovarian cancer: insights into chemotherapy-driven heterogeneity and personalized treatment strategy.
    Article Snippet: .. Combined cells were split and incubated with FcR blocking reagent (Miltenyi Biotec, Adliswil, Switzerland) for 10 min at 4°C before staining for 30 minutes at 4°C with either the tumor or the immune antibody panels. .. Before acquisition on CyTOF (Helios system, Standard BioTools), the cells were resuspended in 1mL of nucleic acid Ir-Intercalator in 1.6% PFA/PBS for 1 h at room temperature (Standard BioTools).

    Article Title: Identification of immunopeptides (pHLA) as candidate therapeutic targets in chondrosarcoma
    Article Snippet: Cell concentration was adjusted to 1 × 10 6 cells/mL in PBS and cells were incubated with viobility dye (VioGreen, Miltenyi Biotec) for 20 min in the dark. .. Cells were then washed twice with FACS buffer (0,1% BSA, 0,5 mM EDTA), incubated with FcR blocking reagent (Miltenyi Biotec) for 15 min at 4 °C and washed twice with FACS buffer. ..

    Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract.
    Article Snippet: In addition, a barcoded anti-human CD27 antibody (TotalSeq-C0154, BioLegend) was included, and BM and gut cell suspensions were labeled separately with anti-human Hashtag 1 (TotalSeqC0251, BioLegend) and Hashtag 2 (TotalSeq-C0252, BioLegend) antibodies, respectively. .. The cells were incubated with FcR blocking reagent (Miltenyi Biotec) prior to addition of antibodies and antigens. ..

    Article Title: A poly(I:C)/QS-21-based in situ vaccine synergizes with anti-PD-1 therapy to overcome tumor immunoresistance.
    Article Snippet: The single-cell suspension Cell Reports Medicine 7, 102932, August 18, 2026 e5 Article ll OPEN ACCESS was lysed with red blood cell lysis buffer (420302, Biolegend). .. Cells were incubated with FcR Blocking Reagent (130-092-575, Miltenyi Biotec) before staining. ..

    Article Title: Poly(I:C) and QS-21 combination suppresses breast tumor growth and metastasis by repolarizing tumor associated macrophages to anti-tumor macrophages.
    Article Snippet: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer defined by a paucity of targeted therapies, high metastatic propensity, and an immunosuppressive tumor microenvironment rich in tumor-associated macrophages (TAMs).. Here, we developed a liposomal immunoadjuvant system co-encapsulating Poly(I:C) and QS-21 aimed at reprogramming TAMs and bolstering antitumor immunity.. In an orthotopic 4T1 TNBC mouse model, intratumoral injection of the combined formulation markedly suppressed primary tumor growth and pulmonary metastasis, with no observable systemic toxicity.

    Incubation:

    Article Title: Humans homozygous for rare or common hypomorphic IL23R variants are prone to tuberculosis
    Article Snippet: .. They were then permeabilized/stained by incubation overnight at −20°C in the permeabilization buffer from the Nuclear Transcription Factor Buffer Set (BioLegend) with an intracellular cytokine panel containing FcR blocking reagent (Miltenyi Biotec; 1:50 dilution), anti-IFN-γ-BV711 (Clone: 4 S.B3, 502540; BioLegend; 1:50 dilution), anti-TNF-BV510 (Clone: MAb11, 502950; BioLegend; 1:50 dilution), and anti-IL-10-PE/Dazzle594 (Clone: JES3-19F1, 506812; BioLegend; 1:50 dilution) antibodies. .. As a positive control, cells in a separate well were stimulated by incubation with PMA (Sigma-Aldrich; 25 ng/ml) and ionomycin (Sigma-Aldrich; 500 nM) for 1 h without GolgiPlug and then for 7 h with GolgiPlug (for intracellular cytokine staining).

    Article Title: A tumor profiling resource for ovarian cancer: insights into chemotherapy-driven heterogeneity and personalized treatment strategy.
    Article Snippet: .. Combined cells were split and incubated with FcR blocking reagent (Miltenyi Biotec, Adliswil, Switzerland) for 10 min at 4°C before staining for 30 minutes at 4°C with either the tumor or the immune antibody panels. .. Before acquisition on CyTOF (Helios system, Standard BioTools), the cells were resuspended in 1mL of nucleic acid Ir-Intercalator in 1.6% PFA/PBS for 1 h at room temperature (Standard BioTools).

    Article Title: Identification of immunopeptides (pHLA) as candidate therapeutic targets in chondrosarcoma
    Article Snippet: Cell concentration was adjusted to 1 × 10 6 cells/mL in PBS and cells were incubated with viobility dye (VioGreen, Miltenyi Biotec) for 20 min in the dark. .. Cells were then washed twice with FACS buffer (0,1% BSA, 0,5 mM EDTA), incubated with FcR blocking reagent (Miltenyi Biotec) for 15 min at 4 °C and washed twice with FACS buffer. ..

    Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract.
    Article Snippet: In addition, a barcoded anti-human CD27 antibody (TotalSeq-C0154, BioLegend) was included, and BM and gut cell suspensions were labeled separately with anti-human Hashtag 1 (TotalSeqC0251, BioLegend) and Hashtag 2 (TotalSeq-C0252, BioLegend) antibodies, respectively. .. The cells were incubated with FcR blocking reagent (Miltenyi Biotec) prior to addition of antibodies and antigens. ..

    Article Title: A poly(I:C)/QS-21-based in situ vaccine synergizes with anti-PD-1 therapy to overcome tumor immunoresistance.
    Article Snippet: The single-cell suspension Cell Reports Medicine 7, 102932, August 18, 2026 e5 Article ll OPEN ACCESS was lysed with red blood cell lysis buffer (420302, Biolegend). .. Cells were incubated with FcR Blocking Reagent (130-092-575, Miltenyi Biotec) before staining. ..

    FACS:

    Article Title: Identification of immunopeptides (pHLA) as candidate therapeutic targets in chondrosarcoma
    Article Snippet: Cell concentration was adjusted to 1 × 10 6 cells/mL in PBS and cells were incubated with viobility dye (VioGreen, Miltenyi Biotec) for 20 min in the dark. .. Cells were then washed twice with FACS buffer (0,1% BSA, 0,5 mM EDTA), incubated with FcR blocking reagent (Miltenyi Biotec) for 15 min at 4 °C and washed twice with FACS buffer. ..

    Single Cell:

    Article Title: Poly(I:C) and QS-21 combination suppresses breast tumor growth and metastasis by repolarizing tumor associated macrophages to anti-tumor macrophages.
    Article Snippet: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer defined by a paucity of targeted therapies, high metastatic propensity, and an immunosuppressive tumor microenvironment rich in tumor-associated macrophages (TAMs).. Here, we developed a liposomal immunoadjuvant system co-encapsulating Poly(I:C) and QS-21 aimed at reprogramming TAMs and bolstering antitumor immunity.. In an orthotopic 4T1 TNBC mouse model, intratumoral injection of the combined formulation markedly suppressed primary tumor growth and pulmonary metastasis, with no observable systemic toxicity.



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